Seite - (000051) - in Biomedical Chemistry: Current Trends and Developments

2. Enoyl-CoA hydratase performs a nucleophilic addition of water to the unsaturated double bond. The hydroxyl (nucleophile) is added exclusively in the β-position to the carbonyl since the enzyme is stereospecific. 3. The formed hydroxymethylene group is oxidised to a carbonyl group with a β-hydroxyacyl-CoA dehydrogenase. This reaction yields an NADH ion, formed from the coenzyme NAD+ acting as the oxidant, and the β-ketoester.