Page - (000116) - in Biomedical Chemistry: Current Trends and Developments

ACN, acetonitrile; APCI, atmospheric pressure chemical ionization; API, atmospheric pressure ionization; Ara-U, 1-ß-D-arabinofuranosyluracil; AUC, area under the curve; BBB, blood-brain barrier; BCRP, breast cancer resistance protein; CE, collision energy; Cl, systemic clearance; DAD, diode array detection; DP, declustering potential; F, absolute bioavailability; FDA, food and drug administration; GOCarb, guanidine oseltamivir carboxylate; HPLC, high performance liquid chromatography; IS, internal standard; MRM, multiple reaction monitoring; K2 EDTA, dipotassium salt of ethylenediaminetetraacetic acid; MRT, mean residence time; NR, not reported; PDA, photodiode array; PP, protein precipitation; QC, quality control samples; SPE, solid phase extraction; TFA, Trifluoroacetic acid; t1/2, half-life time; UHPLC, ultra-high- performance liquid chromatography; UV, ultraviolet; Vd, volume of distribution; Currently, LC/MS and LC/MS/MS take the bulk of the work during pharmacokinetic evaluation in DDD programs (Table 2.1.6), particularly due to the inherent specificity and sensitivity of these techniques, which dramatically increase throughput for the quantitative determination of drugs and metabolites in biological matrices. In addition, these methods reduce the need of exhaustive chromatographic separations prior to