Page - 24 - in Advances in Neuroimmunology

BrainSci. 2016,6, 15 males inboththegreyandwhitematterof thespinalcordat thedaysstudied(Figure3B).Theseresults indicatedthatastrocytic IL-6deficiencydidnot impactmicrogliosis ineither thegreyorwhitematter of thespinalcord. Finally, vasogenesis was analyzed by counting lectin-stained vessels in the spinal cord of EAE-inducedmice(Figure3D,bottom,arrowsandinsets). In femalemice, thenumberofvessels in the whitematterwassignificantlyreducedinAst-IL-6KOmicecomparedtofloxedmiceatboth20–22and 46dpi (Figure3C).Nogenotypicdifferencewasobservedfor thenumberofvessels in thewhitematter formalesor for thenumberofvessels in thegreymatter for femalesandmalesat the timesstudied. Figure 3. (A–C)Results fromGFAPand lectin stainings in the spinal cord (greyandwhitematter) of EAE-induced animals at 20 dpi (for both females andmalesAst-IL6KOandfloxedmice) and 46dpi (females). Numberofmiceper groupas indicated. GFAPoverall immunostaining (A) and thenumber of lectin-positivemicroglia (B) andvessels (C) are shown. ‹ p<0.05 vs. floxedmice. (D)RepresentativeGFAPat100ˆ (top)andlectinat150ˆ (bottom)stainingsof46and20dpi females, respectively.Arrowsindicatevessels. Thediscontinuous lineseparatesgreymatter (left) fromwhite matter (right).All insertsareat400ˆ; at the topthey, showastrocyticmorphology inbothgreyand whitematter, andat thebottom, theyshowavessel. 24