Page - 92 - in Advances in Neuroimmunology

BrainSci. 2016,6, 25 Figure6.Effectsof restraint stressorWCEonamygdalaneuroimmunemRNAs. ForCCL2, therewas amodest,butsignificant,overall effectof treatments (F(2,27)=4.450,p<0.05)withasignificantgroup differencebetweenWCEandstress. Therewerenoothereffectsof treatments forTNFα, IL-1β, orTLR4 mRNAs. **p<0.01comparedwith thestressedgroup.A,B,C, andDdelineatedata forCCL2,TNFα, IL-1βandTLR4mRNAs, respectively, (n=7–11pergroup). 3.5. Effect of theCRF1RAntagonistCP154,526onCorticalCytokinemRNAsFollowingStress Having twice shownpreviously that at CRF1R antagonist blocks cytokine induction arising duringethanolwithdrawal in thecortex [38], thefinalexperiment focusedondeterminingwhether thedrugwouldalsoblock the inductiondue to stress. Figure 7 illustrates the effect ofCP154,526 oncytokinemRNAs in thecortex. Figure7Ashows that stress increasedCCL2(63%,p<0.05)and this effectwasnotblockedbyCP154,526. Similarly, Figure 7B shows thatTNFαwas increasedby stress (41%,p<0.01)andagainthestresseffectwasunalteredbyCP.Finally, theprofileofactionon IL-1βwassimilarasshowninFigure7Cwhichshowsthat IL-1βwassignificantly increasedbystress (55%,p<0.05)andCP154,526 failedtoblockthis induction. 92