Seite - 69 - in Advances in Neuroimmunology

BrainSci. 2016,6, 16 Table3.BodyWeight. Group %Difference Con/Con(n=10) +1.0%˘1.4%† Con/EtOH(n=11) ´6.6%˘2.1%* EtOH/EtOH(n=8) ´8.7%˘1.7%* Adlibitum (n=4) +25.2%˘1.7% *p<0.05vs.Con/Conandad libitum; † p<0.05vs. ad libitumonly. 3.2.OX-42 Immunoreactivity IncreasedbyEtOHExposure OX-42expressionwasexaminedtodeterminewhethermicrogliawere furtherordifferentially activated following a secondbinge exposure. OX-42positive cellswere apparent in all treatment groups,which is consistentwith its constitutive expression inall typesofmicroglia [59]; however, therewas avisiblydistinct increase in immunoreactivity in ethanol treated animals accompanied byanapparentmorphological change. Microglia inethanol animals appeared tohave shorterbut thickenedramificationscomparedwiththecontrolanimals(Figures1B,Cand2B,C).One-wayANOVAs indicatedasignificanteffectoftreatmentintheCA1(F(3,29)=16.81,p<0.0001),CA2/3(F(3,29)=18.34, p<0.0001), andDG(F(3,29)=14.43,p<0.0001)fields (Figure1), aswell as in theentorhinal cortex (F(3,28)=19.01,p<0.0001) (Figure2).Asexpectedbasedonpreviousdata [22],post-hocTukey’s tests indicatedasignificant increase inOX-42density inall ethanol treatedgroups inall subregionsof the hippocampuscomparedwith thecontrolorad libitumgroups. Importantly, theEtOH/EtOHgroup showedgreater immunoreactivity thanCon/EtOHinall regionsanalyzedexcept theDG.Moreover, nodifference inOX-42wasobservedbetweenad libitumanimalsandtheCon/Congroup.Correlations betweenbingemodelparameters (intoxicationbehavior,doseperday, totaldose,BEC,percentweight loss)andOX-42 immunoreactivitywererunwithintheEtOH/EtOHandCon/EtOHgroup,butno significantcorrelationswereobserved(Table4). Figure1.PotentiatedMicroglialActivationintheHippocampusbyRepeatedEthanolExposure.OX-42 (CD11b/c) is upregulated in thehippocampusof ethanol-exposed rats as shown in representative photomicrographsof the (A–C)hippocampaldentategyrus for (B)Con/EtOHand(C)EtOH/EtOH groupscomparedto (A) controls.AnalysisofOX-42 immunoreactivity indicatedthat theEtOH/EtOH grouphadsignificantlymorestaining than theCon/EtOHgroup in the: (D) cornuamonis1 (CA1) and(E) cornuamonis2/3 (CA2/3) regionsbutnot the (F)dentategyrus (DG).Dataexpressedasa percentageofad libitumcontrol (notshown). Imageswere takenat50ˆmagnificationwith insetsat 600ˆmagnification. Scalebar=200μm;inset30μm. *p<0.05comparedtoad libitumandCon/Con groups;#p<0.05comparedtoCon/EtOH. 69