Seite - 73 - in Advances in Neuroimmunology

BrainSci. 2016,6, 16 Figure6. IncreasedTNF-α inEtOH/EtOHGroup.Concentrationsof (A,B) interleukin-10(IL-10)and (C,D) tumornecrosis factor-α (TNF-α)weredeterminedbyELISAinboththehippocampus(A,C)and entorhinalcortex(B,D).NochangeinIL-10wasmeasuredineither thehippocampusortheentorhinal cortex,butat leasta2.7-foldincreaseinTNF-αwasmeasuredinthe(C)hippocampusintheEtOH/EtOH groupcomparedwithallothergroups.However,nochange inTNF-αwasseeninthe(D)entorhinal cortex. *p<0.05comparedtoad libitumandCon/Congroups;#p<0.05comparedtoCon/EtOH. Figure7.CorrelationsofCytokines: (A)Apositivecorrelationbetweenbloodethanolconcentration (BEC) and tumor necrosis factor-α (TNF-α) concentration. Animalswith BECs over 400mg/dL appear tohavean increase inTNF-α. (B)Apositivecorrelationbetweenhippocampalestimatesof microglianumberandbrainderivedneurotrophic factor (BDNF) concentrations in theCon/EtOH group.Adecline in thenumberofmicrogliacells correlatedwithdecreases inBDNFconcentrations. Table5.SelectHippocampalCytokineandGrowthFactorCorrelations. - TNF-α BDNF Parameter Con/EtOH EtOH/EtOH Con/EtOH EtOH/EtOH IntoxicationBehavior S=0.451 S=0.371 S=´0.421 S=0.216 Dose/Day p=´0.525 p=´0.544 p=0.166 p=´0.149 TotalDose p=´0.496 p=´0.355 p=0.160 p=´0.144 BEC p=´0.081 p=0.807* p=0.166 p=0.298 PercentWeightLoss p=0.117 p=0.610 p=0.395 p=´0.473 OX-42+Density p=0.493 p=´0.139 p=0.253 p=´0.254 Iba-1+Cells p=´0.225 p=´0.372 p=0.835* p=0.224 *p<0.05. 73