Seite - 87 - in Advances in Neuroimmunology

BrainSci. 2016,6, 25 Figure 2. Time course of changes in cerebro-corticalmRNAs for CCL2, IL-1β, TNFα, and TLR4 following 1hof restraint stress. Stress elevatedCCL2mRNA in cortex (F(5,32) = 3.82, p=0.008), aneffect thatpeakedat2and4h.Asimilareffectwas foundfor IL-1βmRNA(F(5,48)=5.13,p<0.01) andTNFα (F(5,53) = 8.51, p<0.0001). TLR4mRNAwas elevatedat 4h (F(5,38) = 8.5, p<0.0001). Inall cases, thecytokinemRNAlevelsgraduallyreturnedtocontrol levelsby24h. **p<0.01*p<0.05 comparedwithcontrols that receivednostress (openbars).A,B,C, andDdelineatedata forCCL2, TNFα, IL-1βandTLR4mRNAs, respectively, (n=5–8per timepoint) 2.6. CRFReceptorAntagonistAdministration SubgroupsofCDratswere injectedoncewith theCRF1RantagonistCP154,526 [38,40]orvehicle 15minprior to the start of stress (Figure1C).Thedrugwaspreparedasamicrofinesuspension in 0.5%carboxymethylcelluoseandadministeredintraperitoneallyatadoseof15mg/kginavolume of2mL/kg. 87