Seite - 108 - in Advances in Neuroimmunology

BrainSci. 2017,7, 14 Table3.Expressionprofileof thecytokineandchemokinegenes in theratbrain inresponse toLPS, withandwithoutmorphine tolerance. morphine-tolerant+ saline/placebocontrol+saline placebocontrol+LPS/placebo control+saline morphine-tolerant+ LPS/morphine-tolerant+saline Gene FoldChange** p-value* FoldChange** p-value* FoldChange** p-value* Ccl11 −2.4662 0.023104 −2.8639 0.16576 −2.7346 0.249229 Ccl12 −1.4362 0.646529 2.0214 0.219015 2.1108 0.399119 Ccl2 1.1756 0.521412 23.7845 0.001354 9.1497 0.106064 Ccl5 −1.105 0.472434 1.2909 0.290174 −1.0949 0.766893 Ccl7 −1.0841 0.688167 10.7188 0.000259 6.9528 0.163034 Cxcl1 1.0131 0.944854 36.8609 0 14.4718 0.130008 Cxcl3 1.6208 0.019673 8.1909 0.000076 3.6477 0.170486 Cd40lg −2.2796 0.215753 1.1119 0.704126 −2.4054 0.273798 Ifnb1 1.2519 0.936419 1.6899 0.391302 1.5695 0.513801 Ifng 1.5355 0.290413 1.1698 0.767882 2.2615 0.088346 Il12a 1.05 0.57557 1.1178 0.33855 1.0837 0.456191 Il12b 2.5587 0.26828 8.1055 0.102341 2.077 0.198782 Il18 1.068 0.60015 1.0484 0.707002 −1.1151 0.48359 Il1b −1.2476 0.284501 6.7366 0.001383 3.3136 0.089312 Il33 1.2342 0.054615 1.0407 0.583823 1.1978 0.072613 Il6 2.023 0.45937 12.0303 0.008058 7.0943 0.083969 Irf1 1.1299 0.521156 3.4575 0.000386 3.4104 0.01276 Irf2 −1.1481 0.050782 1.0027 0.916481 1.0556 0.593809 Irf3 −1.2824 0.024657 −1.3225 0.225481 −1.2478 0.088625 Irf4 −1.1491 0.43182 −1.0557 0.846866 1.0263 0.858979 Irf5 −1.1024 0.38296 −1.1468 0.376953 1.0391 0.841777 Irf6 1.1055 0.413463 1.012 0.792025 1.118 0.272222 Tnfsf11 −1.6077 0.800779 −1.362 0.519436 −1.3266 0.965379 Tnfsf14 −1.396 0.243156 −1.8445 0.126355 −1.4278 0.629476 Tnfsf4 −1.0175 0.997398 −1.0887 0.790199 −1.3422 0.420711 *Forp-value, letters inredmeanp<0.05. **ForFoldChange, letters inredmeanfoldchange>2andletters inblue mean the foldchange<−2. For the color lines, : 6–10 folddecrease; : 2–5 folddecrease; : <2 fold; : 2–5 fold increase; : 6–10 fold increase; : 11–30 fold increase; : 31–50 fold increase. Pla ce bo -co ntr ol+ Sa lin e Pla ce bo -co ntr ol+ LP S Mo rp hin e-t ole ran t+S ali ne Mo rp hin e-t ole ran t+L PS -5 0 5 10 15 Il12a Il12b Il18 Il1b Il33 Il6 ** *** Figure3.Cytokinegeneexpression in theratbrain inresponse to lipopolysaccharides (LPS),withand withoutmorphine tolerance. Geneexpressionof interleukins Interleukin (Il)-1β, Il-6, Il-12a, Il-12b, Il-18,andIl-33 in thebrainsof rats,withandwithoutmorphine tolerance, followingani.p. injection ofeither250μg/kgLPSorsaline (n=3–5ratspergroup)wasdeterminedusingaPolymeraseChain Reaction (PCR) array. Datawere calculatedusing theΔΔCTmethod relative to the control group (placebo-control+saline)andarerepresentedasa foldchange. *p<0.05, **p<0.01, ***p<0.001 Similarly,Ccl2,Ccl7,Cxcl1,andCxcl3chemokineexpressionwassignificantly increased24-, 11-, 37-, and8-fold, respectively (p<0.01–0.001), inresponse toLPSinthecontrol rats (placebo-control+ 108