Seite - 107 - in Advances in Neuroimmunology

BrainSci. 2017,7, 14 Table2.Expressionprofileof inflammasome-relateddownstreamsignalinggenes in theratbrain in response to lipopolysaccharides (LPS),withandwithoutmorphine tolerance. Fullnameof thegenes wereprovidedinTableA1. morphine-tolerant+ saline/placebocontrol+saline placebocontrol+LPS/placebo control+saline morphine-tolerant+ LPS/morphine-tolerant+saline Gene FoldChange p-value* FoldChange** p-value* FoldChange** p-value* Bcl2 1.0469 0.863233 1.0586 0.845254 1.2398 0.339246 Bcl2l1 −1.1256 0.01111 1.0572 0.476643 −1.0291 0.671184 Birc2 1.0509 0.586337 −1.0281 0.622487 1.0699 0.531417 Birc3 1.0648 0.587513 20.9356 0.011065 21.5025 0.001873 Cflar 1.0226 0.697346 1.3021 0.004748 1.2155 0.140978 Chuk 1.1867 0.119108 −1.0233 0.754891 1.1593 0.073058 Ciita −1.0617 0.750879 1.0619 0.873363 1.4678 0.39646 Ctsb −1.0009 0.978499 −1.0656 0.617885 −1.1046 0.493204 Fadd −1.1261 0.258884 1.1235 0.252486 1.0306 0.71174 Hsp90aa1 1.2774 0.114349 1.0391 0.743375 1.189 0.149024 Hsp90ab1 1.0752 0.376876 −1.0968 0.305427 1.1579 0.18515 Ikbkb 1.0718 0.550623 −1.0011 0.962992 1.1799 0.201955 Ikbkg 1.0924 0.324904 −1.0402 0.492725 1.1419 0.079202 Irak1 1.1549 0.062648 1.0287 0.755395 1.2213 0.242955 Map3k7 −1.2495 0.002404 −1.1008 0.434596 −1.2497 0.114056 Map3k7ip1 −1.0335 0.664499 −1.1151 0.369226 1.02 0.864431 Map3k7ip2 −1.2869 0.000867 −1.1169 0.456355 −1.3427 0.007785 Mapk1 −1.0527 0.468581 −1.0887 0.159097 1.0316 0.724595 Mapk11 −1.0673 0.530192 −1.0706 0.578016 −1.0633 0.571223 Mapk12 1.1424 0.420684 1.1889 0.372287 −1.0255 0.780819 Mapk13 −1.1629 0.924422 −1.5423 0.355826 1.0504 0.651078 Mapk14 1.0018 0.940725 −1.0318 0.81795 1.0335 0.709962 Mapk3 −1.1294 0.224192 −1.121 0.372958 −1.0495 0.76638 Mapk8 1.0383 0.883566 −1.0215 0.798267 1.0037 0.911563 Mapk9 −1.1252 0.03886 −1.0075 0.87718 −1.0805 0.288977 Mefv 1.2572 0.278807 1.2582 0.463496 1.9192 0.028283 Myd88 1.0211 0.791137 1.0103 0.917416 1.2185 0.03802 Nfkb1 1.1098 0.279116 1.2919 0.089881 1.3323 0.033357 Nfkbia −1.0294 0.835021 3.885 0.001215 3.3744 0.001348 Nfkbib −1.1473 0.330475 −1.0153 0.762037 −1.1167 0.643504 P2rx7 −1.1586 0.428492 1.0742 0.625549 1.0578 0.661519 Panx1 −1.076 0.275575 1.1455 0.406863 1.1101 0.334734 Pea15a −1.0943 0.291531 −1.043 0.625202 −1.0802 0.492135 Pstpip1 −1.1041 0.527175 −1.1018 0.680263 −1.0232 0.87342 Ptgs2 −1.08 0.499364 1.5126 0.000571 1.1762 0.323717 Rage 1.0729 0.478448 −1.1273 0.17423 1.1854 0.16574 Rela 1.0171 0.804744 1.1801 0.417806 1.2504 0.106174 Ripk2 −1.1057 0.237762 1.2913 0.028006 1.1199 0.389288 Sugt1 1.0063 0.918273 −1.1023 0.519396 −1.1264 0.106262 Tirap −1.3098 0.094434 −1.0971 0.535721 1.0809 0.374146 Hsp90b1 1.0475 0.680071 −1.0334 0.69801 −1.0877 0.314466 Traf6 −1.0344 0.915204 −1.0171 0.943304 1.0988 0.437323 Txnip −1.0761 0.70951 −1.0718 0.699702 −1.0187 0.789181 Xiap 1.0572 0.503462 −1.0558 0.471524 1.0089 0.93573 *Forp-value, letters in redmeanp<0.05. **ForFoldChange, letters in redmean foldchange>2and letters in bluemeanthe foldchange<−2. For thecolor line, : 6–10 folddecrease; : 2–5 folddecrease; : <2 fold; : 2–5 fold increase; : 6–10 fold increase; : 11–30 fold increase; : 31–50 fold increase. 3.3. ExpressionProfile of Inflammasome-RelatedChemokineandCytokineGenesafter anLPSChallenge, withandwithoutMorphineTolerance Cytokine and chemokine gene expression in response to LPSwas greater in the control rats (placebo-control + LPS) compared to the morphine-tolerant animals (morphine-tolerant + LPS) (Table 3). The cytokines IL-1β and IL-6were significantly increased 7- and 12-fold, respectively (p<0.01–0.001), inthecontrolratsgivenLPS(placebo-control+LPS),whereas inthemorphine-tolerant group (morphine-tolerant +LPS) the fold changeswerenot statistically significant (3- and7-fold, respectively)comparedto theratsgivensaline (Figure3,Table3). 107